Averroes Laboratories advocates its prestige as one of the leading organizations in the field of diagnostics, health monitoring & product quality evaluation related to in Poultry, Dairy, Livestock, Pets, and Aquaculture sectors.
Contact Info HUM Heights, Ground Floor, Service Road East, Near Sohan Interchange, Islamabad Expressway, Islamabad. +92-51-2619179 +92311-7772452, +92331-7188877, averroeslaboratories@gmail.comPlease enter subscribe form shortcode
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The Averroes Laboratories (ARL) uses various methods to trace veterinary pathogenic bacteriological tests. This covers a Wide field of knowledge comprising veterinary bacteriology, water microbiology and food bacteriology. A wide range of tests is available, part of which was developed by ARL. Every year the bacteriological laboratory carries out thousands of bacteriological tests (this refers to classical culture methods).
Averroes Laboratories (ARL) ) has a keen insight into the health of farmed animals in the Pakistan. It collects information on the pathogens that endanger the livestock and knows their susceptibility for antibiotics. That makes ARL the ideal partner for projects like clinical effectiveness studies, monitoring antibiotic resistance development, and collecting field isolates for antibiotic resistance tests.
In addition to the classic culture methods and microscopic studies, Averroes Laboratories (ARL) uses serological (e.g. ELISA), histological and molecular-biological (PCR) tests to detect pathogens and, if applicable, their toxins. Quicker, more efficient and cheaper methods are used, provided that they comply with the quality requirements. For carrying out ELISA tests, ARL has a laboratory with a great capacity of tests per day. Various materials can be tested, including organs, tissues, milk, blood and feces. Much bacteriological testing takes place in the framework of health and vaccine monitoring programmes.
If applicable, bacteriological identification is followed by a susceptibility test of the isolated pathogens to antibiotics. This is the basis for a therapy with the lowest risk of development of resistance
If no registered vaccine is available for a certain pathogenic bacterium, ARL can produce an autogenous vaccine. That is a made-to-measure, farm-specific vaccine. Producing autogenous vaccines is a unique cooperative product of the pathology and bacteriology departments. After the pathologist has diagnosed that the agent that was cultured after necropsy was the cause of the disease, this bacterium is used for producing a farm-specific vaccine.
Bacteriological tests are carried out at the departments of immunology, molecular biology, histology and bacteriology. They work with classical culture methods, microscopic testing methods, molecular biological techniques (PCR) and various immunological tests. A number of these tests have been developed within Averroes Laboratories (ARL).
The pathogenic micro-organisms are first cultured. For that purpose a representative sample of material, possibly harboring the agent, is inoculated onto a suitable culture medium. The inoculated medium is incubated a certain time at a certain temperature. Selective and non-selective media are used which can be solid culture media, such as blood agar, or fluid media. The incubation period depends on the bacteria in question and varies from mostly one to two days to sometimes seven days for rapidly growing bacteria and up to eight to ten weeks for slowly propagating mycobacteria (EEv1) . The bacterium is finally identified on the basis of morphological and physiological properties of pure cultures, through biochemical testing, or serological testing.
The antibiotic susceptibility testing of the isolated pathogens is the basis for the therapy that the practitioner uses at the farm, with the lowest risk of resistance development. Susceptibility tests are carried out with a microbroth dilution assay.
This type of testing is used to gain a reasonably fast overall view on whether or not the cultivated bacteria are morphologically different. Countless methods for the staining of different bacteria are as follows the Gram stain, Immunofluorescence tests, Acid-fast Stain (Ziehl-Neelsen), and Capsule Staining.
PCR testing is an example of an amplification testing technique known as Polymerase Chain Reaction.This test method is more frequently used in Averroes Laboratories (ARL). and has been incorporated into the normal practice. This principle is based on the idea of using gene amplification of a particular piece of bacterium DNA or RNA. The size and features of the amplified DNA or RNA are referenced and unique to the bacterium that will be identified. It is known as ‘real-time PCR’ which allows obtaining quantitative results, distinguishing a particular bacterium and determining the approximate number of bacteria in the material within one day. PCR is used among other methods for the identification of the following organisms and toxins in ARL; Salmonella ssp, Mycobacterium, Mycoplasma and toxin genes Pasteurella multocida. For some pathogens. It is also helpful to typing in epidemio,ogy in order to differentiate one strain of a certain bacterial species from other.
The most frequent technique which is employed is the Enzyme-linked Immunosorbent Assay (ELISA) which is commonly utilized in order to test for the presence of antibodies against the micro-organisms in the milk or sera of animals. Thus, in this technique antigen-antibody bounding is depicted, which inturn gives a rapid, easy, robust and a very reliable technique. . Averroes Laboratories (ARL) carries out millions of ELISA tests every year.